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Total RNA content in buffalo oocytes and different stages of preimplantation embryos produced in vitro

Indian Agricultural Research Journals

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Title Total RNA content in buffalo oocytes and different stages of preimplantation embryos produced in vitro
 
Creator RAJHANS, RAJIB
KUMAR, G SAI
CHANDRA, VIKASH
MISHRA, ASHISH
SHARMA, G TARU
 
Subject Buffalo; Embryos; Total RNA; Vitrified oocytes
 
Description Total RNA content can be used as a preliminary indicator of intrinsic quality of in vitro matured (IVM) oocytes and preimplantation stage embryos. The present study was conducted to assess the total RNA content of different preimplantation stage buffalo (Bubalus bubalis) embryos and to study the effect of vitrification on the total RNA content of in vitro matured buffalo oocytes. The amount of total RNA in the pools of immature oocytes (IM, 120), in vitro matured oocytes (MO, 120), preimplantation stage embryos 8–16 cell (8–16,47), morula (M, 13), blastocyst (B,7) as well as in the in vitro matured and vitrified oocytes (MV, 120) was quantified spectrophotometrically. Total RNA content (ng/oocyte or embryo) was reduced slightly from immature (2.24±0.40) to matured oocyte (1.66±0.53) followed by a significant (P
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-01-07
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/3117
 
Source The Indian Journal of Animal Sciences; Vol 79, No 10 (2009)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/3117/1184
 
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