Record Details

Molecular cloning, comparative sequence analysis and prokaryotic expression of GRA5 protein of Toxoplasma gondii*

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Molecular cloning, comparative sequence analysis and prokaryotic expression of GRA5 protein of Toxoplasma gondii*
 
Creator SINGH, HARKIRAT
TEWARI, A K
MISHRA, A K
MAHARANA, B R
RAO, J R
RAINA, O K
 
Subject Cloning; Expression; GRA5; Toxoplasma gondii
 
Description Among the several target molecules for sensitive detection of Toxoplasma gondii, dense granule antigens are considered important as these help in growth and multiplication of the organism in the host. The communication deals with the cloning and sequence analysis of 363 bp entire open reading frame (ORF) of GRA5, a dense granule protein, from 2 Indian isolates of T. gondii (Izatnagar and Chennai) as well as the standard RH strain. The sequence comparison analysis revealed 100% homology between the Chennai and Izatnagar isolates, 99.2% homology of RH strain with both the Chennai and Izatnagar isolates and 100% sequence homology of RH strain of T. gondii with the published sequence. The GRA5 protein (mature) was subsequently expressed in prokaryotic expression system. It had molecular size of ~29 kDa and the level of expression was measured as 12% of the total protein. The concentration of the mature recombinant GRA5 protein was 92 μg/ml. Western blot with Ni-NTA anti-histidine HRPase conjugate and known positive serum confirmed the presence and purity of protein by immunoreactivity at the unique ~29 kDa region.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor ICAR
 
Date 2011-03-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/4790
 
Source The Indian Journal of Animal Sciences; Vol 81, No 3 (2011)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/4790/1974
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences