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Occurrence and characterization of Escherichia coli O157:H7 and other serotypes in goat and sheep meat in India

Indian Agricultural Research Journals

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Title Occurrence and characterization of Escherichia coli O157:H7 and other serotypes in goat and sheep meat in India
 
Creator VIJAYARANI, K
PARTHIBAN, M
RAJA, A
KUMANAN, K
 
Subject E. coli 0157:H7; Goats; Molecular characterization; Mutton; Serotyping
 
Description In the present study 307 goat and sheep meat samples were tested for Escherichia coli serotypes, which resulted in 4 Enterohaemorrhagic Escherichia coli (EHEC) from goat meat. All the four isolates produced verotoxin as evident from the in-vitro vero cell assay and the multiplex PCR. Serotyping of these isolates indicated that one isolate belonged to serotype O157 (M2), 2 to serotype 071 (23 and C3) and 1 to 060 (M). Molecular typing also confirmed that, isolate M2 belonged to 0157 serotype with positive amplification for eae A and hly A primers. PCR with H7 gene specific primers, also gave positive amplification with isolate M2, indicating that this isolate belonged to 0157:H7 serotype. Further studies for antibiotic resistance indicated that the E. coli 0157HH7 isolate from goat meat had multiple antibiotic resistance.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor ICAR
 
Date 2010-10-18
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/1518
 
Source The Indian Journal of Animal Sciences; Vol 80, No 10 (2010)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/1518/459
 
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