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Isolation and characterization of buffalo amniotic fluid stem cells

Indian Agricultural Research Journals

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Title Isolation and characterization of buffalo amniotic fluid stem cells
 
Creator ANJANA, P
KUMANAN, K
PRATHIBAN, M
BALASUBRAMANIAM, S
 
Subject Amniotic fluid cell; Buffalo; In-vitro differentiation; Stem cells
 
Description Amniotic fluid (AF) present different cellular types in its composition and a specific group of these cells shows multipotent characteristics with high proliferation ratio and increased differentiation ability. In this study, we isolated and characterized adult stem cells from buffalo (Bubalus bubalis) to check their differentiation potential in-vitro and evaluated their plasticity. Single cell derived stem cell clones were isolated by limiting dilution. AF cells showed both fibroblast and epitheloid morphology. Amniotic fluid stem cells (AFSC) were differentiated into osteogenic, chondrogenic and adipogenic lineage by culturing in specific differentiation medium. Differentiation was confirmed by cell staining with alizarin red S, toluidine blue and oil red O specific for osteogenesis, chondrogenesis and adipogenesis respectively. Gene expression of stem cell markers were found to be positive for Oct- 4, Sox–2 and Nanog by RT- PCR.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2012-06-05
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/18943
 
Source The Indian Journal of Animal Sciences; Vol 82, No 6 (2012)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/18943/9300
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences