Record Details

Fatty acyl chains of phosphatidyl choline and ethanolamine as diacyl, alkyl and alkenyl forms during in vitro capacitation of goat spermatozoa

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Fatty acyl chains of phosphatidyl choline and ethanolamine as diacyl, alkyl and alkenyl forms during in vitro capacitation of goat spermatozoa
 
Creator DHANDA, SUMAN
ATREJA, S K
 
Subject Acrosome reaction; Capacitation; Goat sperm; Phosphatidylcholine; Phosphatidylethanolamine; Phospholipids
 
Description The fluidity of sperm plasma membrane increases during capacitation and acrosome reaction as these processes involve the remodeling of many proteins and phospholipid components. Therefore, we were interested in comparing the fatty acyl chains associated with phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in capacited and acrosome reacted spermatozoa of goat. PC and PE were separated from total phospholipids (PL) during in vitro capacitation and acrosome reaction. Radyl acetates were prepared from diradyl derivatives after phospholipase C treatment of PC and PE in the presence of pyridine and acetic anhydride. The corresponding fatty acyl chains were analysed by gas liquid chromatography (GLC) in diacyl, alkenyl and alkyl radyl acetate by separation and methyl ester preparation. C12, C14: 0, C16: 0, C18: 0, C20: 0 and C24: 0 are saturated and C16: 1, C18: 1, and C20: 4 were the main unsaturated fatty acyl chains. Moreover, the major fatty acyl chains of spermatozoa are different from those of surrounding fluid during the course of in vitro capacitation and acrosome reaction. The results clearly indicated the rapid turnover of these 2 sperm phospholipids in terms of active deacylation and reacylation reactions. These changes would definitely alter the sperm fertilizing ability. This may also generate bioactive PLs, viz. autocoids, fusogens, cis-unsaturated fatty acids, secondary messengers and detergents to accomplish the processes preceeding and succeeding fertilization.m
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-02-09
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/4016
 
Source The Indian Journal of Animal Sciences; Vol 81, No 2 (2011)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/4016/1596
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences