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Vitrification of immature goat oocytes by solid surface vitrification technique

Indian Agricultural Research Journals

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Title Vitrification of immature goat oocytes by solid surface vitrification technique
 
Creator NEMA, SONAL
JAIN, S K
GEHLAUT, B S
SARKHEL, B E
 
Subject Cryopreservation; Goat; Oocytes; Solid surface vitrification; Vitrification
 
Description The immature oocytes were vitrified by solid surface vitrification (SSV) technique. Oocytes were instantly vitrified with minimum quantity (1-2 J.lI) droplets of vitrification medium into a solid metallic surface, partially immersed into liquid nitrogen (LN2).The recovered rate ofnormal oocytes after thawing, cumulus expansion and polar body observed in vitrified-thawed with cumulus (VTe) were significantly higher as compared to vitrified-thawed oocytes without cumulus (VTO). The rate ofrecovered normal oocytes after thawing were 40.23±2.56% and 22.66±1.84% in VTC and VTa group respectively. Similarly polar body observed in VTC and VTO groups were 32.18±2.88% and 15.33±3.05% respectively. However, the cumulus expansion in non-vitrified with cumulus (NVC) group was significantly higher than VTC group (58.73±4.79% vs 32. 18±:2.88%). In line with the values with the cumulus expansion, the polar body development was also significantly high in NVC group as compared to VTC group (57.14±4.64% vs 32.18±4.43%). The high rate of cryosurvival following vitrification by SSV method used in the present study may be due to high . cooling rate, using the combination of microdrops and improved heat exchange achieved by direct contact with metal surface. The technique after further standardization can provide a steady source ofmaterials for in-vitro embryo production and embryo manipulation research. 
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-04-05
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/5484
 
Source The Indian Journal of Animal Sciences; Vol 77, No 7 (2007)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/5484/2271
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences