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Assessment of nuclear maturation and subsequent in vitro embryo development of caprine oocytes with different supplementations in maturation medium

Indian Agricultural Research Journals

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Title Assessment of nuclear maturation and subsequent in vitro embryo development of caprine oocytes with different supplementations in maturation medium
 
Creator YADAV, PARUL
KHARCHE, S D
GOEL, A K
JINDAL, S K
GOEL, PUJA
 
Subject 4', 6-diamidino-2-phenylindole (DAPI), Embryo development, Caprine oocytes, In-vitro fertilization, In-vitro maturation
 
Description The maturation medium and selection of protein supplements, growth factors, antioxidants and hormones for IVM play an important role in subsequent in-vitro fertilization and in-vitro embryo development. The objective of the present experiment was to study the effect of exogenous addition of hormones, epidermal growth factor (EGF), insulin and ßmercaptoethanol into the maturation medium for in-vitro maturation of caprine oocytes. A total of 3753 Cumulus oocyte complexes (COCs) were collected from slaughtered goat ovaries, randomly divided and matured into following treatment groups. Group 1 (control): TCM-199 medium containing 10% newborn calf serum (NCS) and 3mg/ml BSA used as base medium, group 2: base medium supplemented with FSH (5μg/ml), LH (5μg/ml) and estradiol-17ß (1μg/ml), group 3: base medium supplemented with insulin (50ng/ml), group 4: base medium supplemented with FSH (5μg/ml),LH (5μg/ml), estradiol-17ß (1μg/ml) and epidermal growth factor (EGF; 10ng/ml), group 5: base medium supplemented with FSH (5μg/ml), LH (5μg/ml), estradiol-17ß (1μg/ml) and ß-mercaptoethanol (50mM/ml) and group 6: COCs were matured in a base medium supplemented with FSH (5μg/ml), LH (5μg/ml), estradiol-17ß (1μg/ml) and insulin (50 ng/ml). After maturation, 1, 540 oocytes from different groups were assessed for the evidence of nuclear maturation. Remaining 2,213 oocytes from different maturation group were allowed for in vitro fertilization and subsequent in vitro embryo development. The nuclear maturation rates in groups 1 to 6 were 33.6%, 38.0%, 39.7%, 60.0%, 37.4%, and 44%, respectively. The cleavage rate and morula production rate in groups 1 to 6 were 10%, 24%, 27%, 25%, 31%, 28% and 5.12%, 15.6%, 5.55%, 10.41%, 13.3%, 5.49% respectively. Statistical analysis (ANOVA) after arcsin transformation revealed that the maturation rate in group 4 was statistically significant (P
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2013-10-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/33853
 
Source The Indian Journal of Animal Sciences; Vol 83, No 10 (2013)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/33853/15016
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences