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Accelerated In Vitro Propagation of Sweetpotato Clones (Ipomoea batatas L.)

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Title Accelerated In Vitro Propagation of Sweetpotato Clones (Ipomoea batatas L.)
 
Creator Vollmer, R.
Espirilla, J.
Sánchez, J.C.
Arroyo, L.
Flores, G.
Anglin, Noelle L.
Kreuze, J.F.
Ellis, David
 
Subject sweet potatoes
ipomoea batatas
clones
 
Description Fast and reliable propagation of plant material is an important need in different stages of breeding programs and production systems. In vitro propagation ensures that pathogen- and virus-free plants stay phytosanitary clean over time while providing high multiplication rates. Using liquid instead of solid culture medium can reduce the interval of individual propagation cycles and contributes to speeding up of the process (1.5–2.5 times), especially during the initial growth phase of the plants. Sophisticated immersion systems have been developed for many plant species, but they are difficult to apply when hundreds or thousands of different genotypes are propagated simultaneously. Additionally, these systems require a high input of technical equipment, know-how and experience to avoid bacterial or fungal contamination during the propagation process. The following protocol describes a low-input suspension technique that combines the use of liquid and solid medium, and permits the successful propagation of genetically diverse sweetpotato genotypes [Ipomoea batatas (L.) Lam.] with a high multiplication rate. As sweetpotato is an important staple crop in low-income/technology countries of Africa, Asia, and South America, the described method may find valuable application for the breeding programs in these regions.
 
Date 2022-10-06
2022-10-07T19:13:13Z
2022-10-07T19:13:13Z
 
Type Book Chapter
 
Identifier Vollmer, R.; Espirilla, J.; Sánchez, J.C.; Arroyo, L.; Flores, G.; Anglin, N.L.; Kreuze, J.F.; Ellis, D. 2022. Accelerated In Vitro Propagation of Sweetpotato Clones (Ipomoea batatas L.). In: Kamaluddin, Kiran, U., Abdin, M.Z. (eds) Technologies in Plant Biotechnology and Breeding of Field Crops. Springer, Singapore. ISBN: 978-981-16-5767-2. pp 133–149.
9789811657665
9789811657672
https://hdl.handle.net/10568/124952
https://doi.org/10.1007/978-981-16-5767-2_7
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format pp 133–149
 
Publisher Springer Science and Business Media LLC