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Overcoming Challenges for Shoot Tip Cryopreservation of Root and Tuber Crops

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Title Overcoming Challenges for Shoot Tip Cryopreservation of Root and Tuber Crops
 
Creator Zhang, A.
Wang, M.
Li, Z.
Panis, B.
Bettoni, J.C.
Vollmer, R.
Xu, L.
Wang, Q.
 
Subject cassava
cryopreservation
potatoes
root crops
tubers
sweet potatoes
taro
 
Description Root and tuber crops (RTCs) are the second-most important carbohydrate commodity after cereals. Many species of the RTCs are vegetatively propagated, making their shoot tips the preferred material to be conserved for future uses. Shoot tip cryopreservation provides an important tool to support the long-term conservation of plant genetic resources. Over the past four decades, significant efforts have been undertaken to move shoot tip cryopreservation of RTCs from research projects to full-scale implementation in cryobanks. This comprehensive review focuses on the history of cryopreservation protocols developed in RTCs. The encapsulation and vitrification solution-based cryopreservation techniques followed by ultra-rapid freezing and thawing have been highly successful. Additionally, different strategies for improving the cryotolerance of shoot tips have been introduced to further increase post-cryopreservation recovery. Finally, the research conducted to explain the mechanism underlying cryoprotection and differential cryotolerance including the use of histological studies are highlighted.
 
Date 2023-01-11
2023-03-29T17:54:24Z
2023-03-29T17:54:24Z
 
Type Journal Article
 
Identifier Zhang, A.; Wang, M.; Li, Z.; Panis, B.; Bettoni, J. C.; Vollmer, R.; Xu, L.; Wang, Q. 2023. Overcoming Challenges for Shoot Tip Cryopreservation of Root and Tuber Crops. Agronomy. ISSN 2073-4395. 13(1). 219. 37 p.
2073-4395
https://hdl.handle.net/10568/129802
https://doi.org/10.3390/agronomy13010219
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 37 p.
 
Source Agronomy