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Improvement of recovery cycle of cryopreserved potato shoot tips – Small changes, but huge impact

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Title Improvement of recovery cycle of cryopreserved potato shoot tips – Small changes, but huge impact
 
Creator Vollmer, R.
Espirilla, J.
Villagaray, R.
Castro, M.
Cardenas, J.
Pineda, S.
Azevedo, Vania
 
Subject cryopreservation
potatoes
 
Description Improvement and optimization of cryopreservation protocols for its reliable large-scale application is a time-consuming process. The development of robust and efficient protocols implicates the assessment of a high number of diverse genotypes before results can be extrapolated on complete collections. To simplify and improve the recovery cycle of potato shoot tips cryopreserved with the droplet-vitrification method, it was assessed if placing shoot tips after thawing for 9 days on culture medium with normal and stable sucrose concentration of 0.073 M (in darkness), can increase the viability rate, compared to a stepwise decrease of the sucrose concentration from 0.3 M to 0.2 M to 0.1M and 0.073 M during the initial phase of the recovery cycle (control treatment). The experiment was performed with 68 diverse potato landraces which showed a significantly higher recovery rate of 69.8 % when shoot tips were recovered on culture medium with a stable sucrose concentration of 0.073 M, compared to the control treatment (57.9 %). The difference in recovery rates was confirmed during routine cryopreservation: a set of 806 accessions recovered with the control treatment showed an average recovery rate of 60.9 % (years 2018-19), while 1158 accessions processed with the new protocol had an average recovery rate of 75.1 % (2019-now). Currently, CIP conserves 4017 virus-free potato accessions in its cryobank, which represent about 83% of the in vitro collection (4851 accessions). Based on a high experimental sample size of 68 tested accessions (1.4 % of population) robust and reliable results about the cryo-response of the whole collection were obtained.
 
Date 2022-12
2023-03-23T16:46:48Z
2023-03-23T16:46:48Z
 
Type Journal Article
 
Identifier Vollmer, R.; Espirilla, J.; Villagaray, R.; Castro, M.; Cárdenas, J.; Pineda, S.; Azevedo, V. 2022. Improvement of recovery cycle of cryopreserved potato shoot tips – Small changes, but huge impact. Cryobiology, 109, 13–14. https://doi.org/10.1016/j.cryobiol.2022.11.044
1090-2392
https://hdl.handle.net/10568/129750
https://doi.org/10.1016/j.cryobiol.2022.11.044
 
Language es
 
Rights Copyrighted; all rights reserved
Limited Access
 
Format 13-14
 
Source Cryobiology