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Detection of Edwardsiella tarda from fish by the specific amplification of a 450 bp fragment of 168 rRNA gene and its sequencing

Indian Agricultural Research Journals

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Title Detection of Edwardsiella tarda from fish by the specific amplification of a 450 bp fragment of 168 rRNA gene and its sequencing
 
Creator KUMAR, GOKHLESH
SWAMINATHAN, THANGARAJ RAJA
RATHORE, GAURAV
SOOD, NEERAJ
KAPOOR, D
 
Subject Edwardsiella tarda; Fish; PCR; 16S ribosomal RNA gene
 
Description Species-specific primers were developed to detect the 16S ribosomal RNA gene of Edwardsiella tarda. The region of 16S rRNA gene 450 bp was amplified in all 16 isolates of E. tarda. Amplification, which resulted -in detectable levels of PCR product, was achieved when a minimum of 8 CFU/ml of E. tarda. The detection limit for 165 rRNA gene by PCR amplification ofgenomic DNAwas 20 pg. There was no cross reactivity found with Escherichia coli, Salmonella arizonae, Pseudomonas alcaligenes, Aeromonas hydrophila, Vibrio cholerae, Staphylococcus aureus and Flavobacterium spp. 
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-03-08
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/4697
 
Source The Indian Journal of Animal Sciences; Vol 78, No 4 (2008)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/4697/1917
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences