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Inhibitory effects of nitric oxide on steroidogenesis by buffalo granulosa cells cultured in vitro

Indian Agricultural Research Journals

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Title Inhibitory effects of nitric oxide on steroidogenesis by buffalo granulosa cells cultured in vitro
 
Creator SHANMUGAM, M
PANDITA, S
PALTA, P
 
Subject Buffalo, Granulosa cells, L-NAME, Nitric oxide, SNAP, Steroidogenesis
 
Description This study examined the effects of S-nitroso-N-acetyl-penicillamine (SNAP), a nitric oxide (NO) donor and Nw-nitro-L-argininemethyl ester hydrochloride (L-NAME), a nitric oxide synthase inhibitor on estradiol-17β andprogesterone production by buffalo granulosa cells. Granulosa cells (3×105) from small ( 9mm diameter) follicles were cultured for 24 h under completely serum-free conditions in DMEM: nutrient mixture F-12Ham (1:1 ratio) supplemented with 10–7 M androstenedione, 5 mg/ml human apo-transferrin, 0.1% BSA, in the presenceor absence of FSH (8 ng/ml). Granulosa cells from large follicles produced higher estradiol-17β and progesterone thanthose from small follicles. SNAP reduced estradiol-17β and progesterone production at concentrations of 0.1 and 1 mMwhen used alone and in the presence of FSH by granulosa cells from follicles of both size categories. L-NAME (0.2, 1and 5 mM) had, however, no effect on estradiol-17β or progesterone production, alone or in the presence of FSH. Ourresults demonstrated strong inhibitory effects of NO on estradiol-17β and progesterone production by buffalo granulosacells from small and large follicles and indicated that it may be an autocrine/paracrine regulator of steroidogenesis bygranulosa cells in buffalo.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2013-01-16
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/26393
 
Source The Indian Journal of Animal Sciences; Vol 83, No 1 (2013)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/26393/12084
 
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