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Outer membrane protein profiles of Pasteurella multocida isolates of bovine origin

Indian Agricultural Research Journals

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Title Outer membrane protein profiles of Pasteurella multocida isolates of bovine origin
 
Creator Arora, A K
Jand, S K
Sandhu, K S
Oberoi, M S
 
Subject Bovine; Haemorrhagic septicaemia; Immunoblotting; Outer membrane proteins; Pasteurella multocida
 
Description Outer membrane protein (OMP) profiles of 17 different Pasteurella multocida isolates of bovine origin were studied by SDS-PAGE and immunoblotting. The proteins were extracted by sonication, ultra centrifugation and detergent (sodium lauroyl sarcosinate) treatment. All the isolates showed homogenous protein profiles comprising 23 different polypeptide bands ranging in molecular weight from 13 to 94 kDa. On the basis of stain intensity 32 kDa protein appeared to be the major protein band followed by 25 and 28 kDa bands. Other protein bands observed were 13,34, 44.5,46, 80 and 84 kDa. Immunoblotting was carried out using homologous antiserum raised in rabbits after single injection of formalin-killed antigen. The 32 kDa protein was found to be the immunodominant along with 2.5 kDa protein band in all the isolates.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Date 2007-08-05
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/10357
 
Source The Indian Journal of Animal Sciences; Vol 77, No 8 (2007)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/10357/4758
 
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