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Omp31 gene based molecular detection of Brucella melitensis from serum samples of goats

Indian Agricultural Research Journals

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Title Omp31 gene based molecular detection of Brucella melitensis from serum samples of goats
 
Creator SINGH, A
GUPTA, V K
KUMAR, A
SINGH, V K
N, SHIVASARANAPPA
 
Subject Brucellosis, Goat, Omp31gene, Serum PCR
 
Description The present study analyses the sensitivity and specificity of OMP31 gene based amplification directly from sera samples. Sero-positive animals (73) in rOmp31 i-ELISA were subjected to Omp31 gene amplification from sera samples and only 40 samples amplified 723kb product suggesting these as true positive animals. When similar test was applied with the sera samples of 16 infected (culture positive) and 20 non-infected (culture negative) goats, all the culture positive animals revealed 723 kb product while none of the culture negative goats revealed amplification. Hence serum based PCR appeared to be 100% sensitive and specific in truly infected animals. Thus serum is the optimal specimen for the diagnosis of brucellosis and leads to simplification of assay and shortens turnaround time.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2014-03-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/38697
 
Source The Indian Journal of Animal Sciences; Vol 84, No 3 (2014)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/38697/17406
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences