Record Details

Molecular typing of Indian Salmonella isolates from poultry samples

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Molecular typing of Indian Salmonella isolates from poultry samples
 
Creator SIDDIQUE, R A
SAXENA, M
LAKHCHAURA, B D
 
Subject RAPD; PCR; Salmonella; Poultry
 
Description Genus Salmonella consists of an important zoonotic, ubiquitous, organisms causing disease in human as well as animals. In the present study molecular typing of Indian Salmonella isolates, 300 poultry samples (fecal, meat and egg) were screened for Salmonella. Rapidly detected by targeting invA gene, giving PCR product of 284 bp size. Positive samples of Salmonella were serotyped. Five RAPD primers were used to generate randomly amplified polymorphic DNA fragments. RAPD of 24 Salmonella isolates by all 5 primers generated 122 loci. Out of which, 18 were unique and 104 loci were polymorphic. The size of these fragments ranged from 200 bp to 3.0 Kbp. Dendrogram of RAPD for 24 Salmonella isolates revealed similarly between 14.3 to 50%, It is concluded that RAPD technique can be used to demonstrate differences among same serovars of Salmonella.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor icar
 
Date 2011-01-06
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/2790
 
Source The Indian Journal of Animal Sciences; Vol 79, No 4 (2009)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/2790/893
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences