Record Details

Immature buffalo (Bubalus bubalis) oocytes vitrification: Effect of ethylene glycol concentrations on survivability and nuclear maturation

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Immature buffalo (Bubalus bubalis) oocytes vitrification: Effect of ethylene glycol concentrations on survivability and nuclear maturation
 
Creator BOMBATKAR, R S
PAWSHE, C H
CHAUHAN, M S
RAUT, P B
 
Subject Buffalo; Ethylene glycol; In vitro maturation; Oocytes; Vitrification
 
Description The objective of the present research work was to study the effect of 5, 6 and 7 M ethylene glycol (EG) concentrations during vitrification of immature buffalo oocytes. The COCs were retrieved by aspirating visible follicles on the ovaries collected from slaughterhouse and loaded into 0.25 ml French straws after exposing to the equilibration medium (50% VS v/v in DPBS) for 1 min and the final vitrification solutions of respective concentrations of EG. The straws were then directly plunged into the LN2. After thawing in warm water (28–30°C), for equilibration COCs were kept for 4 step dilutions step-wise in 0.5, 0.33, 0.17 and 0.00 M sucrose holding medium for 5 min each. The percent morphologically normal oocytes recovered were significantly higher (75.00%) in 7 M concentration. For nuclear maturation, the COCs were placed in 50 μl droplets of maturation medium and cultured for 24 h in CO2 incubator at 39°C temperature. The proportion of maturation reached to metaphase-II was significantly higher in 7 M (47.42%) concentration whereas the highest nuclear degeneration was in 6 M (70.47%) concentration. From the results, it was revealed that 7 M concentration has beneficial effect on survivability and nuclear maturation of oocytes.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor ICAR
 
Date 2010-07-21
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/309
 
Source The Indian Journal of Animal Sciences; Vol 80, No 7 (2010); 39-43
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/309/291
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences