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Diagnosis of benzimidazole resistance in Haemonchus contortus using allele-specific PCR technique

Indian Agricultural Research Journals

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Title Diagnosis of benzimidazole resistance in Haemonchus contortus using allele-specific PCR technique
 
Creator GARG, RAJAT
YADAV, C L
 
Subject Allele specific PCR; benzimidazole resistance; Haemonchus contortus
 
Description Molecular tools can be of great use in understanding the origin and nature of underlying mechanisms of benzimidazole resistance. In the present study an allele-specific PCR was standardized to diagnose the mutation (Phe to Try) at residue 200 of the isotype 1 beta tubulin gene responsible for benzimidazole resistance in Haemonchus contortus adult and infective larvae. Once standardized, AS-PCR proved to be a powerful tool to detect point mutations at codon 200 of isotype 1 ß-tubulin gene of H. contortus adult worms and infective larvae. The technique can be effectively used for genotyping of benzimidazole susceptible and resistant alleles of H. contortus from sheep.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-01-07
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/3113
 
Source The Indian Journal of Animal Sciences; Vol 79, No 10 (2009)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/3113/1180
 
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