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Cloning, expression and phylogenetic analysis of IFN-γ gene in chicken

Indian Agricultural Research Journals

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Title Cloning, expression and phylogenetic analysis of IFN-γ gene in chicken
 
Creator CHATURVEDI, UTTARA
KALIM, SHAHINA
SINGH, LAKSHYA VEER
SAHOO, A P
PALIA, S K
KUMAR, RAJIV
KUMAR, G RAVI
TIWARI, SANGEETA
TIWARI, ASHOK K
 
Subject Genetic adjuvant, IFN γ, RT-PCR
 
Description In the present study, chicken interferon gamma (Ch.IFN-γ) gene was amplified using specific primers by RT-PCRand cloned into pTZ 57R/T cloning vector. The clone was confirmed by restriction digestion analysis using KpnI andApaI restriction enzymes and orientation was confirmed by colony PCR using T7 promoter primer and gene specificforward primers. Further, the gene fragment was sub-cloned in eukaryotic expression vector pcDNA 3.1(+) and confirmedby restriction digestion. The recombinant pcDNA 3.1 having IFN gene (pcDNA.Ch-IFN-γ) was checked for its abilityto express IFN-γ in vitro in cell culture and expression was confirmed by RT-PCR, indicating the gene is functionallyactive. The study to evaluate ability of pcDNA.Ch-IFN-γ to combat infection and enhance efficacy of DNA vaccine isunder way.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2013-04-10
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/28925
 
Source The Indian Journal of Animal Sciences; Vol 83, No 4 (2013)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/28925/13084
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences