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Some studies on immunological characterization of sheep hydatid fluid antigens of Echinococcus granulosus

Indian Agricultural Research Journals

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Title Some studies on immunological characterization of sheep hydatid fluid antigens of Echinococcus granulosus
 
Creator Pal, S
Singh, B P
 
Subject SDS-PAGE (sodium dodceyl sulphate polyacrylamide gel eleetrophroesis); SHCF (sheep hydatid cystic fluid)
 
Description The sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) resolved the crude sheep hydatid cystic fluid (SHCF) into 5 molecular moieties having molecular weights of 67, 45, 42, 32 and 24 kDa with an increasing mobility. Thc hydatid cystic fluid was subjected to sephadex G 150-120 column chromatography and different fractions were collected. First pooled sample (P₁) showed only one band of 67 kDa molecular weight (m.w.), whereas second pooled sample (P₂) showed 2 polypeptides of 67 and 32 kDa m.w. and third (P₃) showed a 32 kDa m.w. polypeptied band on SDS-PAGE. Rest of the pooled gel filtrated fractions revealed no polypeptide band.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2012-07-18
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/21369
 
Source The Indian Journal of Animal Sciences; Vol 69, No 11 (1999)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/21369/10809
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences