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Prediction of fertilization potential of frozen spermatozoa of cattle and buffalo

Indian Agricultural Research Journals

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Title Prediction of fertilization potential of frozen spermatozoa of cattle and buffalo
 
Creator KUMAR, SUBODH
SHARMA, ARJAVA
 
Subject Buffalo, Cattle, Fertility, Platelet activating factor, Prediction equation
 
Description Frozen semen of 12 bulls each of Karan Fries cattle and Murrah buffalo of varying conception rates (CR), after appropriate thawing and washing, was subjected to 200 inM platelet activating factor (PAF) treatment for 15 min at 38°C and 5% CO2 leveL Thereafter treated spermatozoa were subjected to zona free hamster oocyte penetration test and fertilization parameters, viz. sperm attached per ovum (SA/O), ova attached (OA), fertilization per cent (FP), fertilization index (FI) and polyspermic ova (PO) were evaluated. R1 values were highest for SA/O and FI in both the species. Regressing them jointly against observed CR, the values of intercept constant (a), regression coefficients (b1 and b) for SA/O and Fl were evaluated as 25.0615, 1.1706 and -2.9245 for cattle and -61.9162, 0.0771 and 93.2561 for buffalo. CR was predicted by fitting these values in simple linear regression equation Y=a+b1X1+b2X2, which, was significantly (P
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2014-01-09
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/36555
 
Source The Indian Journal of Animal Sciences; Vol 71, No 4 (2001)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/36555/16282
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences