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Sex determination in ratite and non ratite birds by molecular method

Indian Agricultural Research Journals

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Title Sex determination in ratite and non ratite birds by molecular method
 
Creator BHATT, RISHABH
PATEL, A C
JISHA, T K
MACWAN, SHEFALI
THAKER, RIDHDHI
RANK, D N
 
Subject Emu, Gender differentiation, HRM, Non ratite, Ostrich, PCR, Ratite
 
Description In spite of number of methods for sex determination in birds, it is very difficult to distinguish sex especially in ratite birds due to lack of sexual dimorphism. Chromodomain helicase DNA binding 1 gene (CHD 1) is the choice of gene for gender differentiation using PCR based molecular method. In present study, non ratite CHD gene specific primers viz. 1237L/1272H, 2550F/2718R, P2/P8, P2/P3 and ratite bird specific primers viz.W5/ W7 andW1/ K7 were used for gender differentiation in ratite birds. The ratite bird specific primer W5/W7 was the only primer, which determined the sex in emu as well as ostrich successfully, while 1237L/1272H, 2550F/2718R, P2/P8, P2/P3 primers were unable to discriminate sex in emu and ostrich but ratite and non ratite primers can be used to discriminate the sex in non-ratite bird, primarily in chicken. In an alternative approach of PCR-RFLP, the high resolution melting curve (HRM) analysis showed conflicting pattern in both sexes of ratite birds but in chicken HRM analysis showed clear cut differential melting temperature in both sexes, hence HRM can be used for gender differentiation successfully.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2016-02-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/55800
 
Source The Indian Journal of Animal Sciences; Vol 86, No 2 (2016)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/55800/23469
 
Rights Copyright (c) 2016 The Indian Journal of Animal Sciences