Synchronization of ovulation and subsequent fertility in buffaloes followingPGF2α-PGF2α protocol, with or without GnRH
Indian Agricultural Research Journals
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Title |
Synchronization of ovulation and subsequent fertility in buffaloes followingPGF2α-PGF2α protocol, with or without GnRH
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Creator |
DADARWAL, D
GHUMAN, S P S HONPARKHE, M SINGH4, JAGIR |
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Subject |
Buffalo;corpus luteurn; Ovulation synchronization ; Progesterone; Prostaglandin
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Description |
A PGF2α-based protocol, with or without GnRH, was used to synchronize ovulation and assess subsequent fertility in cycling buffaloes. In protocol-A (n=6) and protocol-B (n=6), buffaloes were administered, at unknown stage of estrous cycle, 2 PGF2α injections (1 mg cloprostenol each) 12 days apart, whilst buffaloes of protocol-B received an additional GnRH injection (0.02 mg buserelin) at 48 h after second-PGF2α. Buffaloes were artificially inseminated (AI) twice at 48 and 72 h after second-PGF2α. The luteal profile of day 0 reached basal values in all the buffaloes at 72 h subsequent to first-PGF2α on day 0. The ovulatory follicles observed on day 0, ovulated between 48–120 h subsequent to first-PGF2α in 92 per cent buffaloes. In response to second-PGF2α on day 12, all the buffaloes exhibited luteal regression and displayed onset of estrus around 24 h after second-PGF2α. Ovulation in response to second-PGF2α induced luteolysis was observed in all the buffaloes between 72–96 h after second-PGF2α. Conception of buffaloes was similar across treatments at 42nd day (50%). The administration of GnRH in protocol-B had no effect on time of ovulation or conception. In summary, 2 injections of PGF2α administered 12 day apart in cycling buffaloes were highly efficacious for synchronizing ovulation and thus, permitting fixed-time AI at 72 h after second-PGF2α which results in good enough fertility
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Publisher |
Directorate of Knowledge Management in Agriculture
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Contributor |
icar
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Date |
2011-01-05
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Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion — |
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Format |
application/pdf
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Identifier |
http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/2639
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Source |
The Indian Journal of Animal Sciences; Vol 79, No 9 (2009)
0367-8318 |
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Language |
eng
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Relation |
http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/2639/754
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Rights |
Copyright (c) 2014 The Indian Journal of Animal Sciences
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