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Extracellular and cell-bound verocytotoxins of Salmonella serovars

Indian Agricultural Research Journals

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Title Extracellular and cell-bound verocytotoxins of Salmonella serovars
 
Creator SINGH, Y
SHARMA, V D
CHANDRA, R
GARG, S K
 
Subject Salmonella serovars, Vero cell-line, Verocytotoxicity
 
Description The 27 strains of Salmonella belonging to 9 different serovars, namely S. Enterica sub. sp. Enterica serovar Newport, S. stanley, S. weltevreden, S. typhimurium, S. senftenberg, S. agona, S. bareilly, S. braenderup and S. kentucky isolated from foods of animal origin, were tested for their cytotoxicity on Vera cell-line. The cell sonicates of all the 27 strains of Salmonella showed variable degree of cytotoxicity, while 25 CFCS (92.5%) of these strains were found cytotoxic to Vero cells. The intensity of changes in Vero cells appeared to be dose dependent and increased irreversibly with increase in incubation period. Polymyxin B sulphate (@0.25 mglml in PBS, pH 7.2), urea (8 M in tris-HCI buffer, pH 8.2) and cell -sonication facilitated the release of cytotoxin.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2013-11-07
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/34874
 
Source The Indian Journal of Animal Sciences; Vol 67, No 8 (1997)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/34874/15415
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences