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Platelet activating factor-A new phospholipid for improving in vitro fertilizing ability of cattle spermatozoa

Indian Agricultural Research Journals

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Title Platelet activating factor-A new phospholipid for improving in vitro fertilizing ability of cattle spermatozoa
 
Creator KUMAR, SUBODH
SHARMA, ARJAVA
 
Subject Cattle, Fertilization parameters, Platelet activating factor, Spermatozoa, Zona free hamster test
 
Description Crossbred cattle (12; Karan Fries) bulls were selected on the basis of their conception rate. Frozen semen was procured, thawed and treated with 200 uM platelet activqting factor (PAF) for 15 min at 38°C and 5% CO2, level. Comparison was made with controls (ie. not treated with PAF.). The controls and treated samples were evaluated for motility, acrosome reaction (AR) and zona free hamster oocyte penetration test to assay fertilizational parameters, viz. sperm attached per ovum (SA/O), ova attached (OA), fertilization per cent (FP), fertilization index (FI) and polyspermic ova (PO). The paired t-test revealed that PAF had a significant (at different levels) effect on all the parameters. All the parameters except motility showed an increasing trend post-PAF treatment.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2014-01-27
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/37020
 
Source The Indian Journal of Animal Sciences; Vol 71, No 10 (2001)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/37020/16553
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences