Record Details

Effect of different activation protocol on generation of parthenogenetic embryos in caprine

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Effect of different activation protocol on generation of parthenogenetic embryos in caprine
 
Creator PANKAJ, R S
SATISHA, K B
VENKATESH, V
GUPTA, R
KUMAR, K
DAS, B C
MAJUMDAR, A C
BAG, S
RANJAN, R
 
Subject Caprine; Cytochalasin B; Ionomycin; Ethanol; Parthenogenetic embryo
 
Description The present study was carried out to compare the different activation protocol for generation of parthenogenetic embryos in caprine. For parthenogenetic embryo production, 6 activation protocols, viz. 7% ethanol in modified synthetic oviductal fluid (mSOF) for 5 min (P-1), 5μM ionomycin in mSOF for 5 min (P-II), 27 h maturation in maturation media containing 10 and 20 μg/ml cytochalacin B (CCB) followed by activation with (a) 7% ethanol in mSOF for 5 min (P-III, P-IV), or (b) 5μM ionomycin in mSOF for 5 min (P-V and P-VI). All these treatments were followed by 4 h incubation with 2mM 6-dimethylaminopurine (6-DMAP) in mSOF. The activated oocytes were further cultured in mSOF for embryo development. The cleavage rate was higher when oocytes were activated by ionomycin than ethanol but further embryo development was better in ethanol activated oocytes. When oocytes were matured in presence of CCB followed by ionomycin or ethanol activation, the cleavage rate and embryo development did not change significantly and was almost similar to when oocytes were activated simply by ethanol or ionomycin. The study indicated that ethanol and ionomycin alone or even in combination with cytochalacin B induced activation and parthenogenetic development of in vitro matured caprine oocytes.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2012-11-19
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/25136
 
Source The Indian Journal of Animal Sciences; Vol 82, No 11 (2012)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/25136/11696
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences