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Detection and quantification of Salmonella on chicken egg shell by real time PCR

Indian Agricultural Research Journals

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Title Detection and quantification of Salmonella on chicken egg shell by real time PCR
 
Creator SINGH, LAKHAN PAL
YADAV, A S
SINGH, R P
PANDEY, N K
DANGI, S S
SAXENA, R
 
Subject Chicken eggs, invA gene, Real-time PCR (RT-PCR), Salmonella
 
Description Quantification of Salmonella on chicken eggs collected      from selected poultry farms and marketing channels located in  and around Bareilly was carried out using RT-PCR targeting invA gene of Salmonella. RT-PCR conditions were standardised optimizing reaction efficiency and R2 value and the specificity of the desired amplified product was assessed using melting curve analysis which was found to be 82°C for Salmonella. Out of 200 chicken eggs, 8 (4%) were positive for  Salmonella. Out of 100 eggs which were collected from poultry farms, 3 (3%) eggs were positive for the Salmonella while out of 100 eggs from marketing channels, 5 (5%)   eggs were found positive for the Salmonella. The level of Salmonella cells per egg was higher (2.186 × 10,4 to 3.039 × 106 CFU/egg) in positive eggs collected from marketing channels than eggs collected from poultry farms (2.08 × 103 to 3.62 × 103 CFU/egg). The results indicated RT-PCR technique as a quick and sensitive method of quantification of salmonella on chicken egg shell surface.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2013-05-10
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/29733
 
Source The Indian Journal of Animal Sciences; Vol 83, No 5 (2013)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/29733/13379
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences