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Genetic polymorphism of melatonin receptor 1A (MTNR1A) gene in Indian sheep breeds

Indian Agricultural Research Journals

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Title Genetic polymorphism of melatonin receptor 1A (MTNR1A) gene in Indian sheep breeds
 
Creator MEENA, A S
KUMAR, RAJIV
KUMARI, RAJNI
JYOTSANA, BASANTI
PRINCE, L L L
KUMAR, SATISH
 
Subject DNA polymorphism, Melatonin receptor 1A, Reproduction, Sheep
 
Description Polymorphism in the melatonin receptor 1A (MTNR1A) is associated with seasonal reproduction of sheep. Genetic polymorphism in MTNR1A gene was investigated in 4 Indian sheep breeds reared in semi-arid and arid region of the Rajasthan. In this study, blood samples were collected from 171 animals. DNA was extracted from white blood cells. The PCR product (824bp) of exon II fragment of sheep MTNR1A gene was amplified. PCR products were digested separately with 2 restriction endonucleases, viz. MnII and RsaI. Polymorphism was detected at both loci of MTNR1A gene. At MnII locus, allele frequency for Magra, Garole, Malpura and Avikalin breed was found as 0.74, 0.74, 0.78, and 0.84 for M allele and 0.26, 0.26, 0.22, 0.16 for N allele, respectively. At RsaI locus, allele frequency was found as 0.90, 0.83, 0.69, and 0.59, for R allele and 0.10, 0.17, 0.31, 0.41 for r allele, respectively. It was concluded that the frequencies of ‘M’ and ‘R’ alleles of MTNR1A were higher in Indian sheep breeds.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2013-12-19
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/35803
 
Source The Indian Journal of Animal Sciences; Vol 83, No 12 (2013)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/35803/15846
 
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