Record Details

Evaluation of pre-erythrocytic stage recombinant proteins of Theileria annulata for early diagnosis of bovine tropical theileriosis in Indian cattle

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Evaluation of pre-erythrocytic stage recombinant proteins of Theileria annulata for early diagnosis of bovine tropical theileriosis in Indian cattle
 
Creator A, VANLALHMUAKA
BANSAL, G C
SARAVANAN, B C
RAO, J R
RAY, D D
 
Subject Bovine tropical theileriosis; ELISA; India; Recombinant; Serodiagnosis; SPAG–1; TaSP; Theileria annulata
 
Description A study was carried out to evaluate 2 pre-erythrocytic recombinant proteins for the diagnosis of bovine tropical theileriosis during the initial phase of the disease. Theileria annulata surface protein (TaSP) and the C terminal region of SPAG–1 were cloned and expressed as fusion with thioredoxine in E. coli. The proteins were purified to near homogeneity using Ni-NTA agarose affinity chromatography. In ELISA, recombinant TaSP (rTaSP) was able to detect antibodies from 7 to 14 days onward in all the 4 experimental bovine calves while no significant results were obtained with rSPAG–1 (C-terminus). rTaSP also showed higher sensitivity than crude piroplasmic antigen in 50 field sera by ELISA. In addition, rTaSP did not show any cross reactivity with Babesia bigemina and Trypanosoma evansi positive sera. The present findings indicated rTaSP as a potential molecule for serodiagnosis of T. annulata.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor ICAR
 
Date 2010-09-09
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/841
 
Source The Indian Journal of Animal Sciences; Vol 80, No 9 (2010)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/841/345
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences