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Random amplified polymorphic DNA (RAPD) analysis ofMycobacterium bovis strain in India

Indian Agricultural Research Journals

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Title Random amplified polymorphic DNA (RAPD) analysis ofMycobacterium bovis strain in India
 
Creator Singh, J P N
Verma, Rishendra
Chaudhuri, P
 
Subject India, Mycobacterium bovis, Random primer, RAPD, Typing
 
Description The usefulness of random amplification of polymorphic DNA (RAPD) analysis for typing Indian strains of Mycobacterium bovis was mvestigated. M bovis ANs' M. bovis BCG and 20 field strains of M. bovis were subjected to RAPD-PCR Using 7 random decamer primers. All 7 primers were differentiative and produced specific RAPD profiles. The polymorphic amplication served as RAPD markers for M. bovis. The dendrograms, obtained by different primers, showed the discrimatory ablity of the rinmers. RAPD analysis provided a rapid and easy means of identifying polymorphIsm M. bovis and It was a valuable alternative epidemiological tool. In addition, the results ofthe present study showed heterogeneIty mthe M bovis strains in the population studied. 
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor
 
Date 2011-03-03
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/4455
 
Source The Indian Journal of Animal Sciences; Vol 76, No 11 (2006)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/4455/1754
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences