Record Details

Isolation of immunoreactive proteins from the larval extracts of Boophilus microplus and Hyalomma anatolicum anatolicum

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Isolation of immunoreactive proteins from the larval extracts of Boophilus microplus and Hyalomma anatolicum anatolicum
 
Creator GHOSH, S
KHAN, M H
 
Subject Boophilus microplus, Gel filtration, Hyalomma a anatolicum, Larval antigen, Proteins
 
Description Crude larval antigens of Boophilus microplus (LEB) and Hyalomma a. anatolicum (LMS) were purified by gel filtration and anion-exchange chromatography to isolate the immunoreactive antigens responsible for induction of resistance. Gel filtration and DEAE-sepharose chromatography of LEB yielded 120 and 76 fractions, respectively, but only one collected by 0.2 M NaCl showed significant precipitation reactions. Similarly, by sephadex G-200 filtration followed by anionexchange chromatography of LMS, an immunoreactive fraction was isolated. Sodium dodecyl sulphate polyacrylamide gel electrophoresis of reactive fractions showed a 68 kDa protein in LEB and 62 and 29 kDa proteins in LMS. The study presents physicochemical procedures for the purification of tick proteins.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2013-11-28
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/35443
 
Source The Indian Journal of Animal Sciences; Vol 70, No 1 (2000)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/35443/15671
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences