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Molecular cloning and characterization of alpha lactalbumin gene of Vechur cattle

Indian Agricultural Research Journals

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Title Molecular cloning and characterization of alpha lactalbumin gene of Vechur cattle
 
Creator RAJEEV, M
ARAVINDAKSHAN, T V
 
Subject Alpha-lactalbumin; Cattle; DNA sequencing; Molecular cloning; Protein structure; Vechur cattle
 
Description A 1756 bp fragment of the alpha-lactalbumin (α-LA) gene of Vechur cattle covering the entire transcriptional unit was PCR-amplified from genomic DNA and was sequenced by the dideoxynucleotide method after cloning in a Tvector. The Vechur α-LA gene has an open reading frame of 426 nucleotides encoding a signal peptide of 19 amino acid residues and a mature protein of 123 amino acid residues with NH2-terminal glutamic acid and COOH- terminal leucine (Accession # EU 200932). Sequence analysis showed that 122 amino acid residues are identical with that of Bos taurus α-LA and 73% identity was observed with human α-LA protein. The nucleotide sequence of Vechur cattle α-LA gene was having 99% homology with that of Bos taurus, 98% with that of yak and 95% with that of sheep α-LA gene. The exon-intron boundaries are conserved in Vechur and Bos taurus as well as in human. This strict homology in the sites of insertion of introns suggests that the exon-intron organization of these genes was established before the divergence of these species. The positions of the exon-intron boundaries are also conserved as evidenced from similar sizes of the exons. The degree of structural or functional similarity between Vechur and human α-LA proteins was almost similar to the similarity between Bos taurus and human α-LA proteins.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor ICAR
 
Date 2010-06-24
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/149
 
Source The Indian Journal of Animal Sciences; Vol 80, No 6 (2010)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/149/141
 
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