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Plasma growth hormone profile in buffalo neonates

Indian Agricultural Research Journals

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Title Plasma growth hormone profile in buffalo neonates
 
Creator Jailkhani, Sujata
Madan, M L
 
Subject Buffalo neonates; Plasma growth hormone
 
Description A sensitive radioimmunoassay (RIA) procedure for estimation of growth hormone in buffalo plasma using highly purified bovine GH was developed, Radioiodination of the hormone was carried out with carrier free 125N-I. Immune reaction was performed using buffalo plasma, buffer, labelled antigen and the antiserum at room temperature. Separation of antibody-bound GH from free GH was accomplished with goat antirabbit gamma globulin. The calibration curve was sensitive from 19.5 pg to 50 ng/ml. Using this procedure, mean plasma concentration of GH was found to be significantly high (P
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2012-07-02
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/20442
 
Source The Indian Journal of Animal Sciences; Vol 69, No 4 (1999)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/20442/10384
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences