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In-vitro proliferation of Musa balbisiana improves with increased vitamin concentration and dark culturing

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Title In-vitro proliferation of Musa balbisiana improves with increased vitamin concentration and dark culturing
 
Creator Ssekiwoko, F.
Talengera, D.
Kiggundu, A.
Namutebi, M.K.
Karamura, E.B.
Kunert, Karl J.
 
Subject antioxidants
ascorbic acid
disease resistance
drought resistance
musa balbisiana
 
Description Musa balbisiana is a wild banana genotype with important traits such as drought tolerance and disease resistance. Uniform and clean plants are often required to study these traits in different laboratories but plants can only be generated through a tissue culture process yet for a long time a protocol for regeneration of the same has not been available. Here, we demonstrate that modification of the anti-oxidant content of the in- vitro plant proliferation medium through adjusting the concentration of ascorbic acid and thiamine HCl in the basal MS medium together with subjecting the explants to dark culturing conditions improved proliferation of M. balbsiana by over 10 fold. These treatments resulted in 40 shoots per initial explant material at the best performance.
 
Date 2014
2015-05-13T13:59:59Z
2015-05-13T13:59:59Z
 
Type Journal Article
 
Identifier Ssekiwoko, F.; Talengera, D.; Kiggundu, A.; Namutebi, M.K.; Karamura, E.; Kunert, K. -2014-In-vitro proliferation of Musa balbisiana improves with increased vitamin concentration and dark culturing-Journal of Applied Biology & Biotechnology 2(3)-p. 001-007
2347-212X
https://hdl.handle.net/10568/66072
http://www.ejmanager.com/mnstemps/125/125-1406619457.pdf?t=1407241127
 
Rights Open Access
 
Format p. 001-007
application/pdf
 
Publisher Open Science Publishers LLP (India)
 
Source Journal of Applied Biology & Biotechnology