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Chromosome configurations of buffalo oocytes matured in-vitro

Indian Agricultural Research Journals

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Title Chromosome configurations of buffalo oocytes matured in-vitro
 
Creator Das, S K
Chauhan, M S
Palta, P
Tomer, O S
 
Subject Buffalo; Chromosomes; In-vitro maturation; Oocytes
 
Description Oocytes aspirated from slaughterhouse buffalo ovaries were cultured in-vitro. After 24 hr of culture the oocytes were fixed and stained with Giemsa stain and analyzed for chromosome configuration. The appearance of various chromosome configurations was documented. Out of 125 oocytes, 25 (20%), 3 (2.4%), 4 (3.2%), 9 (7.2%), 1 (0.8%), 2 (1.6%), 16 (12.8%) and 65 (52.0%) oocytes were at germinal vesicle, condensing chromosome-I, condensing chromosome-II, early diakinesis, diakinesis, anaphase-I, telophase-I and metaphase-II stages, respectively. When oocytes at telophase-I and metaphase-II were pooled to express nuclear maturation, 64.8% oocytes showed nuclear maturation in-vitro.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2012-07-06
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/21306
 
Source The Indian Journal of Animal Sciences; Vol 69, No 10 (1999)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/21306/10787
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences