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Level of extra and intracellular enzymes during preservation of good and poor quality ejaculates in crossbred bulls

Indian Agricultural Research Journals

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Title Level of extra and intracellular enzymes during preservation of good and poor quality ejaculates in crossbred bulls
 
Creator GEBRESELASSIE, G A
SRIVASTAVA, S K
GHOSH, S K
TRIPATHI, R P
 
Subject Crossbred, Ejaculate, Enzyme, Semen, Spermatozoa
 
Description Activities of superoxide dismutase (SOD), catalase (CAT) as extracellular and glutamate pyruvate transaminase (GPT), lactic dehydrogenase (LDH) as well as glutamate oxaloacetate transaminase (GOT) as intracellular enzyme were estimated in seminal plasma of good and poor quality ejaculates of crossbred bull semen. SOD, LDH and GOT activities differed significantly in both types of ejaculates at pre-freeze and post- thaw levels, whereas no difference was observed in catalase activity. The GPT activity was also higher in seminal plasma at post-thaw than prefreeze level in both types of ejaculates but it was less compared to other enzymes. Although there was loss in SOD activity during freezing thawing in both type of ejaculates but it was more in poor quality (62.5%) than good quality (50.4%), which may be due to the higher percentage of dead spermatozoa during freeze thaw in former than later. LDH and GOT enzyme activity also increased in seminal plasma in both types of ejaculates after post-thaw but it was higher in poor quality, which indicated that damage in sperm plasma membrane was more in poor quality during freezing thawing.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2013-07-10
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/31325
 
Source The Indian Journal of Animal Sciences; Vol 83, No 7 (2013)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/31325/14060
 
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