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Immunodominant polypeptides in somatic antigen of adult Haemonchus contortus by western blotting and immunoprecipitation

Indian Agricultural Research Journals

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Title Immunodominant polypeptides in somatic antigen of adult Haemonchus contortus by western blotting and immunoprecipitation
 
Creator PRASAD, A
NASIR, A
SINGH, NEM
 
Subject Haemonchus contortus; Immunoaffinity chromatography; Immunoprecipitation;
 
Description Haemonchus contortus commonly found in sheep and goat is a pathogenic abomasal nematode. Adult worms collected from abomasums of infected animals were utilized for the preparation of somatic antigen and purified through immunoaffinity chromatography. Purified fraction was analysed through SDS-PAGE and immunodominant polypeptides were identified through western blotting. On probing with experimental sera of sheep infected with 8000 L3 of H contortus 60 and 120 kDa polypeptides were recognised as immunodominant polypeptides. In immunoprecipitation, first to eight week sera ofsheep captured H contortus antigen which upon elution from the immune complex revealed 60 and 120 kDa polypeptides in SDS-PAGE analysis. The study indicated that 60 and 120 kDa polypeptides were immunodominant in somatic antigen of adult H. contortus which may be utilized further for immunodiagnosisl immunoprophylactic purposes in H contortus infection. 
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-04-05
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/5480
 
Source The Indian Journal of Animal Sciences; Vol 77, No 7 (2007)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/5480/2267
 
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