Record Details

A study on kappa casein gene in water buffaloes using polymerase chain reaction and sequence specific oligonucleotide probes (PCR-SSOP)

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title A study on kappa casein gene in water buffaloes using polymerase chain reaction and sequence specific oligonucleotide probes (PCR-SSOP)
 
Creator MALIK, S
KUMAR, SATISH
RANI, RAJNI
 
Subject K-casein; Milk protein polymorphism; Murrah; PCR-SSOP.
 
Description Polymorphism of Kappa casein gene was studied in genomic DNA samples isolated from peripheral blood lymphocytes of 24 animals of Murrah breed of buffaloes using polymerase chain reaction and sequence specific oligonucleotide probes. The primer and probes for the amplification and hybridization of the variable fragments of the CASK gene was designed from the published nucleotide sequence of the cattle. PCR amplification of buffalo DNA samples and probe hybridization confirmed the homology between the two species at nucleotide level. Inspite of homology, buffalo DNA samples did not show any polymorphism, instead monomorphism was observed at CASK gene. However, in kappa casein gene an interesting hybridization pattern was observed. The kappa casein gene shows monomorphism but it is like CASK-A allele of cattle at codon 136 i.e. ACC (Thr) and like B allele of cattle at codon 148 i.e. GCT (Ala) at the nucleotide level confirming the earlier report on protein sequence.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-08-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/8802
 
Source The Indian Journal of Animal Sciences; Vol 81, No 8 (2011)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/8802/3958
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences