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Modified method for generating microaerophilic environment for isolation of Campylobacter fetus

Indian Agricultural Research Journals

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Title Modified method for generating microaerophilic environment for isolation of Campylobacter fetus
 
Creator Joshi, K
Sharma, N S
Jand, S K
Oberoi, M S
 
Subject Bovine campylobateriosis, Campylobacter fetus; Isolation, Microaerophilic
 
Description Campylobacter fetus is microaerophilic as it requires 5% O2 10 % CO2, and 85 % N , for isolation and cultivation. A modified method was standardised for isolation of Campylobacter fetus. The folumtion, The 80 preputial washing and 50 semen samples in this study, were processed for isolation of Campylobacter fetus by using this method. Sodium bicarbonate, citric acid and sodium borohydride were incorporated in this modified method of creation of microaerophilic environment. The  15 isolates of C.fetus were isolated from 80 preputial washings. All the biochemical tests for identification of these isolated were done under microaerophilic environment. In the present study. 36 samples were also screened by candle jar method and they showed no growth. When these samples were preocessed under microawerophilic environment created by this modifies method, 7 out of 36 samples revealed growth of C. fetus.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-01-21
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/3661
 
Source The Indian Journal of Animal Sciences; Vol 74, No 12 (2004)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/3661/1510
 
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