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PCR based bovine preimplantation embryo sexing: Avoiding false males

Indian Agricultural Research Journals

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Title PCR based bovine preimplantation embryo sexing: Avoiding false males
 
Creator Sood, S K
Madan, M L
 
Subject Bovine; Contamination; Embryo; PCR; Sexing
 
Description Preselection of sex of an offspring in livestock industry has commercial importance as economic value of each livestockspecies is different sex. Polymerase chain reaction (PCR)-based sexing assay has been applied widely to determine sex ofbovine preimplantation embryos. PCR is a powerful technique having potential to amplify even a single copy of target DNA.Therefore, PCR-based sexing assay is vulnerable to contamination and may give a signal of false male. In this review, thecurrent status of PCR and its application to embryo sexing is being reported. Also critical tips to be kept in mind whilesetting up PCR based embryo sexing assay to avoid contamination (hence false males) are enumerated.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2012-06-14
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/19949
 
Source The Indian Journal of Animal Sciences; Vol 68, No 3 (1998)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/19949/10124
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences