Record Details

Generation and characterization of three monoclonal antibodies useful in detecting and distinguishing bean golden mosaic virus isolates

CGSpace

View Archive Info
 
 
Field Value
 
Title Generation and characterization of three monoclonal antibodies useful in detecting and distinguishing bean golden mosaic virus isolates
 
Creator Cancino, M
Abouzid, AM
Morales, Francisco José
Purcifull, DE
Polston, JE
Hiebert, E
 
Subject phaseolus vulgaris
geminiviruses
testa
monoclonal antibodies
nucleotide sequence
geminivirus
anticuerpos monoclonales
secuencia nucleotídica
 
Description Two monoclonal antibodies prepared to purified virions of bean golden mosaic virus (BGMV) isolates from Guatemala (GA) and the Dominican Republic (DR) and one monoclonal antibody prepared to the coat protein of a BGMV isolate from Brazil (BZ) expressed in Escherichia coli were useful serological probes for detection of whitefly-transmitted geminiviruses. Abutilon mosaic, BGMV isolates from Puerto Rico (PR), and Homestead, Florida (H), BGMV-BZ, -DR, and -GA isolates, euphorbia mosaic, rhynchosia mosaic, squash leaf curl, soybean yellow mosaic, an isolate from Macroptilium lathyroides in Florida, and tomato mottle geminiviruses all reacted efficiently in Western blot and enzyme-linked immunosorbent assay with monoclonal antibody 3F7, indicating that this monoclonal antibody has a broad spectrum of reactivity. Another monoclonal antibody (2G5) reacted only with BGMV-DR, -GA, and -PR isolates. A third monoclonal antibody (5C5) reacted only with BGMV-BZ. A new geminivirus infecting cabbage (Brassicaceae) in Florida did not react with any of the three monoclonal antibodies.
 
Date 1995
2014-09-24T08:42:11Z
2014-09-24T08:42:11Z
 
Type Journal Article
 
Identifier 0031-949X
https://hdl.handle.net/10568/43478
https://doi.org/10.1094/Phyto-85-484
 
Language en
 
Rights Open Access
 
Publisher Scientific Societies
 
Source Phytopathology