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Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP)

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Title Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP)
 
Creator Nkere, C.K.
Oyekanmi, J.O.
Silva, G.
Bömer, M.
Atiri, G.I.
Onyeka, J.
Maroya, N.G.
Seal, S.E.
Kumar, P. Lava
 
Subject yams
viruses
plant viruses
plant condition
plant diseases
production
 
Description A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the amplification products in YMV-positive samples are visualized by chromogenic detection with SYBR Green I dye. The CT-RT-LAMP assay detected YMV in leaf and tuber tissues of infected plants. The assay is 100 times more sensitive in detecting YMV than standard RT-PCR, while maintaining the same specificity.
 
Date 2018-04
2020-07-03T08:48:56Z
2020-07-03T08:48:56Z
 
Type Journal Article
 
Identifier Nkere, C.K., Oyekanmi, J.O., Silva, G., Bömer, M., Atiri, G.I., Onyeka, J., ... & Kumar, P.L. (2018). Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP). Archives of Virology, 163(4), 1057-1061.
0304-8608
https://hdl.handle.net/10568/108663
https://doi.org/10.1007/s00705-018-3706-0
BIOTECH & PLANT BREEDING
PLANT PRODUCTION & HEALTH
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 1057-1061
application/pdf
 
Publisher Springer Science and Business Media LLC
 
Source Archives of Virology