Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP)
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Title |
Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP)
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Creator |
Nkere, C.K.
Oyekanmi, J.O. Silva, G. Bömer, M. Atiri, G.I. Onyeka, J. Maroya, N.G. Seal, S.E. Kumar, P. Lava |
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Subject |
yams
viruses plant viruses plant condition plant diseases production |
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Description |
A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the amplification products in YMV-positive samples are visualized by chromogenic detection with SYBR Green I dye. The CT-RT-LAMP assay detected YMV in leaf and tuber tissues of infected plants. The assay is 100 times more sensitive in detecting YMV than standard RT-PCR, while maintaining the same specificity.
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Date |
2018-04
2020-07-03T08:48:56Z 2020-07-03T08:48:56Z |
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Type |
Journal Article
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Identifier |
Nkere, C.K., Oyekanmi, J.O., Silva, G., Bömer, M., Atiri, G.I., Onyeka, J., ... & Kumar, P.L. (2018). Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP). Archives of Virology, 163(4), 1057-1061.
0304-8608 https://hdl.handle.net/10568/108663 https://doi.org/10.1007/s00705-018-3706-0 BIOTECH & PLANT BREEDING PLANT PRODUCTION & HEALTH |
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Language |
en
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Rights |
CC-BY-4.0
Open Access |
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Format |
1057-1061
application/pdf |
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Publisher |
Springer Science and Business Media LLC
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Source |
Archives of Virology
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