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Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL

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Title Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL
 
Creator Jie Song
Dengan Xu
Yan Dong
Faji Li
Bian, Yingjie
Lingling Li
Xumei Luo
Fei, Shuaipeng
Lei Li
Cong Zhao
Yong Zhang
Xianchun Xia
Zhongfu Ni
He Zhonghu
Shuanghe Cao
 
Subject quantitative trait loci
wheat
yields
improvement
genomes
marker-assisted selection
 
Description Thousand kernel weight (TKW) is an important yield component trait in wheat, and identification of the underlying genetic loci is helpful for yield improvement. We previously identified a stable quantitative trait locus (QTL) QTKW.caas-5DL for TKW in a Doumai/Shi4185 recombinant inbred line (RIL) population. Here we performed fine mapping of QTKW.caas-5DL using secondary populations derived from 15 heterozygous recombinants and delimited the QTL to an approximate 3.9 Mb physical interval from 409.9 to 413.8 Mb according to the Chinese Spring (CS) reference genome. Analysis of genomic synteny showed that annotated genes in the physical interval had high collinearity among CS and eight other wheat genomes. Seven genes with sequence variation and/or differential expression between parents were predicted as candidates for QTKW.caas-5DL based on whole-genome resequencing and transcriptome assays. A kompetitive allele-specific PCR (KASP) marker for QTKW.caas-5DL was developed, and genotyping confirmed a significant association with TKW but not with other yield component traits in a panel of elite wheat cultivars. The superior allele of QTKW.caas-5DL was frequent in a panel of cultivars, suggesting that it had undergone positive selection. These findings not only lay a foundation for map-based cloning of QTKW.caas-5DL but also provide an efficient tool for marker-assisted selection.
 
Date 2022-07-29
2023-02-26T17:36:13Z
2023-02-26T17:36:13Z
 
Type Journal Article
 
Identifier Song, J., Xu, D., Dong, Y., Li, F., Bian, Y., Li, L., Luo, X., Fei, S., Li, L., Zhao, C., Zhang, Y., Xia, X., Ni, Z., He, Z. and Cao, S. 2022. Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL. Theoretical and Applied Genetics 135(9):3237–3246.
1432-2242
https://hdl.handle.net/10568/129062
https://doi.org/10.1007/s00122-022-04182-0
 
Language en
 
Rights Copyrighted; all rights reserved
Limited Access
 
Format p. 3237-3246
 
Publisher Springer Science and Business Media LLC
 
Source Theoretical and Applied Genetics