Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL
CGSpace
View Archive InfoField | Value | |
Title |
Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL
|
|
Creator |
Jie Song
Dengan Xu Yan Dong Faji Li Bian, Yingjie Lingling Li Xumei Luo Fei, Shuaipeng Lei Li Cong Zhao Yong Zhang Xianchun Xia Zhongfu Ni He Zhonghu Shuanghe Cao |
|
Subject |
quantitative trait loci
wheat yields improvement genomes marker-assisted selection |
|
Description |
Thousand kernel weight (TKW) is an important yield component trait in wheat, and identification of the underlying genetic loci is helpful for yield improvement. We previously identified a stable quantitative trait locus (QTL) QTKW.caas-5DL for TKW in a Doumai/Shi4185 recombinant inbred line (RIL) population. Here we performed fine mapping of QTKW.caas-5DL using secondary populations derived from 15 heterozygous recombinants and delimited the QTL to an approximate 3.9 Mb physical interval from 409.9 to 413.8 Mb according to the Chinese Spring (CS) reference genome. Analysis of genomic synteny showed that annotated genes in the physical interval had high collinearity among CS and eight other wheat genomes. Seven genes with sequence variation and/or differential expression between parents were predicted as candidates for QTKW.caas-5DL based on whole-genome resequencing and transcriptome assays. A kompetitive allele-specific PCR (KASP) marker for QTKW.caas-5DL was developed, and genotyping confirmed a significant association with TKW but not with other yield component traits in a panel of elite wheat cultivars. The superior allele of QTKW.caas-5DL was frequent in a panel of cultivars, suggesting that it had undergone positive selection. These findings not only lay a foundation for map-based cloning of QTKW.caas-5DL but also provide an efficient tool for marker-assisted selection.
|
|
Date |
2022-07-29
2023-02-26T17:36:13Z 2023-02-26T17:36:13Z |
|
Type |
Journal Article
|
|
Identifier |
Song, J., Xu, D., Dong, Y., Li, F., Bian, Y., Li, L., Luo, X., Fei, S., Li, L., Zhao, C., Zhang, Y., Xia, X., Ni, Z., He, Z. and Cao, S. 2022. Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL. Theoretical and Applied Genetics 135(9):3237–3246.
1432-2242 https://hdl.handle.net/10568/129062 https://doi.org/10.1007/s00122-022-04182-0 |
|
Language |
en
|
|
Rights |
Copyrighted; all rights reserved
Limited Access |
|
Format |
p. 3237-3246
|
|
Publisher |
Springer Science and Business Media LLC
|
|
Source |
Theoretical and Applied Genetics
|
|