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Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

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Title Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)
 
Creator Manoharan, R.
Tripathi, J.N.
Tripathi, L.
 
Subject dioscorea rotundata
somatic embryogenesis
picloram
activated charcoal
white yam
 
Description Dioscorea rotundata, commonly known as white yam, is an important staple food crop widely cultivated in West Africa and provides food security to millions of people. Genetic improvements of this crop using the advanced biotechnology tools have been hampered hitherto by the recalcitrant nature of regeneration through somatic embryogenesis. Therefore, we have developed an efficient and reproducible system for plant regeneration via somatic embryogenesis. Explants of different types (immature leaf, node, stem internode, root segment, petiole, and axillary bud) of D. rotundata accession TDr 2436 were tested for their embryogenic potentials on Murashige and Skoog (MS) medium supplemented with various auxins (2,4-D, NAA, and picloram). Among all explants tested, axillary bud explants cultured on MS medium supplemented with picloram (0.5–12 mg/l) favored the production of calli. Maximum proliferation of calli (526 mg fresh weight/explant) was achieved on MS medium supplemented with picloram (0.5 mg/l), casein hydrolysate (600 mg/l), and proline (1 g/l). Histology analysis confirmed that the embryogenic calli produced on this medium were mixed with non-embryogenic calli. Transfer of calli on MS basal medium supplemented with activated charcoal (1 %) changed the color of calli to purple and promoted the production of somatic embryos (87 embryos/callus) as well as adventitious shoot buds. Furthermore, upon transfer to MS medium supplemented with BAP (0.4 mg/l), the embryos continued their differentiation and maturation and germinated into complete plantlets. The adventitious shoot buds produced multiple shoots on MS medium supplemented with BAP (0.4 mg/l). Well-developed germinated plantlets were acclimatized in the screen house with 90 % survivability. Histology studies confirmed that the regeneration of D. rotundata reported here followed dual regeneration pathways. The embryogenic calli regenerated through development of somatic embryos and germinated into complete plantlets, however non-embryogenic calli regenerated through organogenesis and developed multiple shoots. The developed protocol has potential for somatic hybridization, mass clonal propagation, and genetic transformation applications.
 
Date 2016-09
2016-08-09T17:29:55Z
2016-08-09T17:29:55Z
 
Type Journal Article
 
Identifier Manoharan, R., Tripathi, J., & Tripathi, L. (2016). Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata). Plant Cell, Tissue and Organ Culture,
0167-6857
https://hdl.handle.net/10568/76366
https://doi.org/10.1007/s11240-016-1017-2
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 1-17
application/pdf
 
Publisher Springer Science and Business Media LLC
 
Source Plant Cell, Tissue and Organ Culture