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Chromosome painting facilitates anchoring reference genome sequence to chromosomes In Situ and integrated karyotyping in banana (Musa Spp.)

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Title Chromosome painting facilitates anchoring reference genome sequence to chromosomes In Situ and integrated karyotyping in banana (Musa Spp.)
 
Creator Šimoníková, D.
Němečková, A.
Karafiátová, M.
Uwimana, B.
Swennen, Rony L.
Doležel, Jaroslav
Hřibová, E.
 
Subject bananas
chromosomes
karyotypes
musa
dna
genomes
 
Description Oligo painting FISH was established to identify all chromosomes in banana (Musa spp.) and to anchor pseudomolecules of reference genome sequence of Musa acuminata spp. malaccensis “DH Pahang” to individual chromosomes in situ. A total of 19 chromosome/chromosome-arm specific oligo painting probes were developed and were shown to be suitable for molecular cytogenetic studies in genus Musa. For the first time, molecular karyotypes of diploid M. acuminata spp. malaccensis (A genome), M. balbisiana (B genome), and M. schizocarpa (S genome) from the Eumusa section of Musa, which contributed to the evolution of edible banana cultivars, were established. This was achieved after a combined use of oligo painting probes and a set of previously developed banana cytogenetic markers. The density of oligo painting probes was sufficient to study chromosomal rearrangements on mitotic as well as on meiotic pachytene chromosomes. This advance will enable comparative FISH mapping and identification of chromosomal translocations which accompanied genome evolution and speciation in the family Musaceae.
 
Date 2019
2019-12-19T09:41:18Z
2019-12-19T09:41:18Z
 
Type Journal Article
 
Identifier Šimoníková, D., Němečková, A., Karafiátová, M., Uwimana, B., Swennen, R., Doležel, J. & Hřibová, E. (2019). Chromosome painting facilitates anchoring reference genome sequence to chromosomes in situ and integrated karyotyping in banana (Musa spp.). Frontiers in Plant Science, 10, 1-12.
1664-462X
https://hdl.handle.net/10568/106239
https://doi.org/10.3389/fpls.2019.01503
BIOTECH & PLANT BREEDING
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 1-12
application/pdf
 
Publisher Frontiers Media SA
 
Source Frontiers in Plant Science