Record Details

Molecular epidemiology of Theileria parva in the field

CGSpace

View Archive Info
 
 
Field Value
 
Title Molecular epidemiology of Theileria parva in the field
 
Creator Geysen, D.
Bishop, Richard P.
Skilton, Robert A.
Dolan, T.T.
Morzaria, S.P.
 
Subject theileria parva
epidemiology
dna
molecular markers
genotypes
 
Description Molecular tools based on seminested RFLP-PCR techniques to characterize field parasites in bloodspots dried on filter paper permitted investigation of the extent and the dynamics of diversity of Theileria parva populaitons in the field. Parallel molecular studies explored the long-term genome stability of various isolates by probing Southern blots of EcoRI digested total genomic DNA with four different reference nuclei acid probes. Three polymorphic single copy loci encoding for antigen genes were developed for seminested PCR detection in order to apply them for a multilocus approach in population genetic studies. Seven alleles were identified for the polymorphic immunodominant molecule (PLM) locus by using restriction enzymes, and 4 alleles each for the p150 and p104 loci. A simple DNA extraction method gave good results in amplifying these loci from carrier animals using samples of blood dried on filter papers. Results from probing Southern blots of cultures taken at sequential timepoints indicate relative genome stability in T. parva in comparison to other parasitic protozoa such as Plasmodium. Comapratively homogeneous profiles in sympatric isolates from Zambia were identified using all four probes and PCR amplified products which contrasted with the variety found amongst Kenyan stocks. Preliminary characterization of T. parva field samples from the Southern Province of Zambia strongly suggest clonal expansion of one of the components of a non-Zambian trivalent vaccine used on a limited scale in the Province from 1985 until 1992.
 
Date 1999-09
2013-06-11T09:24:55Z
2013-06-11T09:24:55Z
 
Type Journal Article
 
Identifier Tropical Medicine & International Health;4(9): A21-A27
1360-2276
https://hdl.handle.net/10568/29794
https://doi.org/10.1046/j.1365-3156.1999.00447.x
 
Language en
 
Rights Copyrighted; all rights reserved
Limited Access
 
Publisher Wiley
 
Source Tropical Medicine and International Health