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Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM)

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Title Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM)
 
Creator Skilton, Robert A.
Bishop, Richard P.
Wells, C.W.
Spooner, P.R.
Gobright, E.I.
Nkonge, C.
Musoke, A.J.
Macklin, M.D.
Iams, K.P.
 
Subject vaccines
animal diseases
livestock
 
Description To identify the genes encoding novel immunodominant antigens of Theileria parvo a ?gt11 library of piroplasm genomic DNA was immunoscreened with bovine recovery serum and a gene encoding a 150 kDa antigen (p150) was identified. The predicted polypeptide contains an N-terminal secretory signal sequence and a proline-rich region of repeated amino acid motifs. The repeat region is polymorphic between stocks of T. parva in both copy number and sequence, and analysis of the repeat region from 10 stocks of T. parva revealed 5 p150 variants. A monoclonal antibody (mAb) against the T. parva polymorphic immunodominant molecule (PIM) cross-reacted with the recombinant p150. The p150 has sequence homology with a PIM peptide sequence containing the anti-PIM mAb epitope. Immunoelectron microscopy demonstrated that the p150 antigen, like PIM, is located in the microspheres of the sporozoites and is exocytosed following sporozoite invasion of the host lymphocyte. By immunoelectron microscopy p150 was subsequently transiently detectable on the sporozoite surface and in the lymphocyte cytosol. Immunoblotting showed that p150 is also expressed by the schizont stage, but at much lower levels compared to the sporozoite. These results suggest a major role for p150 in the early events of host-sporozoite interaction.
 
Date 1998-10
2014-04-14T10:56:02Z
2014-04-14T10:56:02Z
 
Type Journal Article
 
Identifier Skilton, R.A., Bishop, R.P., Wells, C.W., Spooner, P.R., Gobright, E., Nkonge, C., Musoke, A.J., Macklin, M. and Iams, K.P. 1998. Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM). Parasitology 117(4): 321-330.
311820
https://hdl.handle.net/10568/35337
https://doi.org/10.1017/S0031182098003163
 
Rights Limited Access
 
Format p. 321-330
 
Publisher Cambridge University Press (CUP)
 
Source Parasitology