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Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells

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Title Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells
 
Creator Lambson, B.
Nene, Vishvanath
Obura, M.
Shah, Tushaar
Pandit, P.
Ole-MoiYoi, O.K.
Delroux, K.
Welburn, S.C.
Skilton, Robert A.
Villiers, Etienne P. de
Bishop, Richard P.
 
Subject ixodidae
metastigmata
salivary glands [exocrine glands]
secretion
mammals
cells
dna
rhipicephalus appendiculatus
nucleotide sequence
amblyomma variegatum
polymorphism
proteins
 
Description Ixodid ticks manipulate mammalian host pathways by secreting molecules from salivary glands. Novel cDNAs containing functional secretion signals were isolated from ixodid tick salivary glands using a signal sequence trap. Only 15/61 Rhipicephalus appendiculatus and 1/7 Amblyomma variegatum cDNAs had significant identity (< 1e-15) to previously identified sequences. Polypeptides that may interact with host pathways included a kinase inhibitor. Two proteins encoded homologues of the yolk protein vitellogenin and seventeen contained glycine-rich motifs. Four proteins without sequence matches had conserved structural folds, identified using a Threading algorithm. Predicted secretion signals were between fifteen and fifty-seven amino acids long. Four homologous polymorphic proteins contained conserved (26/27 residues) signal peptides. Ten functional tick secretion signals could not be unambiguously identified using predictive algorithms.
 
Date 2005-08
2013-07-03T05:25:49Z
2013-07-03T05:25:49Z
 
Type Journal Article
 
Identifier Insect Molecular Biology;14(4): 403-414
0962-1075
https://hdl.handle.net/10568/32949
https://doi.org/10.1111/j.1365-2583.2005.00571.x
 
Language en
 
Rights Copyrighted; all rights reserved
Limited Access
 
Format p. 403-414
 
Publisher Wiley
 
Source Insect Molecular Biology