Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells
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Title |
Identification of candidate sialome components expressed in ixodid tick salivary glands using secretion signal complementation in mammalian cells
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Creator |
Lambson, B.
Nene, Vishvanath Obura, M. Shah, Tushaar Pandit, P. Ole-MoiYoi, O.K. Delroux, K. Welburn, S.C. Skilton, Robert A. Villiers, Etienne P. de Bishop, Richard P. |
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Subject |
ixodidae
metastigmata salivary glands [exocrine glands] secretion mammals cells dna rhipicephalus appendiculatus nucleotide sequence amblyomma variegatum polymorphism proteins |
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Description |
Ixodid ticks manipulate mammalian host pathways by secreting molecules from salivary glands. Novel cDNAs containing functional secretion signals were isolated from ixodid tick salivary glands using a signal sequence trap. Only 15/61 Rhipicephalus appendiculatus and 1/7 Amblyomma variegatum cDNAs had significant identity (< 1e-15) to previously identified sequences. Polypeptides that may interact with host pathways included a kinase inhibitor. Two proteins encoded homologues of the yolk protein vitellogenin and seventeen contained glycine-rich motifs. Four proteins without sequence matches had conserved structural folds, identified using a Threading algorithm. Predicted secretion signals were between fifteen and fifty-seven amino acids long. Four homologous polymorphic proteins contained conserved (26/27 residues) signal peptides. Ten functional tick secretion signals could not be unambiguously identified using predictive algorithms.
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Date |
2005-08
2013-07-03T05:25:49Z 2013-07-03T05:25:49Z |
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Type |
Journal Article
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Identifier |
Insect Molecular Biology;14(4): 403-414
0962-1075 https://hdl.handle.net/10568/32949 https://doi.org/10.1111/j.1365-2583.2005.00571.x |
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Language |
en
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Rights |
Copyrighted; all rights reserved
Limited Access |
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Format |
p. 403-414
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Publisher |
Wiley
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Source |
Insect Molecular Biology
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