Record Details

Analyses of extended-spectrum-β-lactamase, metallo-β-lactamase, and AmpC-β-lactamase producing Enterobacteriaceae from the dairy value chain in India

CGSpace

View Archive Info
 
 
Field Value
 
Title Analyses of extended-spectrum-β-lactamase, metallo-β-lactamase, and AmpC-β-lactamase producing Enterobacteriaceae from the dairy value chain in India
 
Creator Dey, Tushar K.
Lindahl, Johanna F.
Lundkvist, Å.
Grace, Delia
Deka, Ram P.
Shome, R.
Bandyopadhyay, S.
Goyal, N.K.
Sharma, Garima
Shome, B.R.
 
Subject antimicrobial resistance
dairying
infectious diseases
biochemistry
microbiology
 
Description The consumption of milk contaminated with antibiotic-resistant bacteria poses a significant health threat to humans. This study aimed to investigate the prevalence of Enterobacteriaceae producing β-lactamases (ESBL, MBL, and AmpC) in cow and buffalo milk samples from two Indian states, Haryana and Assam. A total of 401 milk samples were collected from dairy farmers and vendors in the specified districts. Microbiological assays, antibiotic susceptibility testing, and PCR-based genotyping were employed to analyze 421 Gram-negative bacterial isolates. The overall prevalence of β-lactamase genes was 10% (confidence interval (CI) (7–13)), with higher rates in Haryana (13%, CI (9–19)) compared to Assam (7%, CI (4–11)). The identified β-lactamase genes in isolates were blaCMY, blaMOX, blaFOX, blaEBC, and blaDHA, associated with AmpC production, while blaCTX-M1, blaSHV, and blaTEM were detected as ESBL producers. While blaVIM, blaIMP, blaSPM, blaSIM, and blaGIM were identified as MBL producers. Notably, Shigella spp. were the dominant β-lactamase producers among identified Enterobacteriaceae. This study highlights the presence of various prevalent β-lactamase genes in milk isolates, indicating the potential risk of antimicrobial-resistant bacteria in dairy products. The presence of β-lactam resistance raises concern as this could restrict antibiotic options for treatment. The discordance between genotypic and phenotypic methods emphasizes the necessity for comprehensive approaches that integrate both techniques to accurately assess antibiotic resistance. Urgent collaborative action incorporating rational and regulated use of antibiotics across the dairy value chain is required to address the global challenge of β-lactam resistance.
 
Date 2023-09-14
2023-09-18T08:00:20Z
2023-09-18T08:00:20Z
 
Type Journal Article
 
Identifier Dey, T.K., Lindahl, J.F., Lundkvist, Å., Grace, D., Deka, R.P., Shome, R., Bandyopadhyay, S., Goyal, N.K., Sharma, G. and Shome, B.R. 2023. Analyses of extended-spectrum-β-lactamase, metallo-β-lactamase, and AmpC-β-lactamase producing Enterobacteriaceae from the dairy value chain in India. Antibiotics 12(9): 1449.
2079-6382
https://hdl.handle.net/10568/131881
https://doi.org/10.3390/antibiotics12091449
https://www.mdpi.com/article/10.3390/antibiotics12091449/s1
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 1449
 
Publisher MDPI
 
Source Antibiotics