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Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids

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Title Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids
 
Creator Zhou, Y.
Zhang, J.
Sun, H.
Tao, D.
Xu, B.
Han, X.
Ren, R.
Ruan, J.
Steinaa, Lucilla
Hemmink, Johanneke D.
Han Jianlin
Li, X.
Xu, J.
Zhao, S.
Xie, S.
Zhao, C.
 
Subject african swine fever virus
diagnostic techniques
 
Description The development of a contamination-free and on-site nucleic acid detection platform with high sensitivity and specificity but low-cost for the detection of pathogenic nucleic acids is critical for infectious disease diagnosis and surveillance. In this study, we combined the recombinase-aided amplification (RAA) with the exonuclease III (Exo III)-assisted signal amplification into a platform for sensitive and specific detection of nucleic acids of African swine fever virus (ASFV). We found that this platform enabled a naked eye visual detection of ASFV at a detection limit as low as 2 copies/μL in 30 min. As expected, no cross-reactivity was observed with other porcine viruses. In addition, to avoid aerosol contamination, a one-tube RAA-Exo III colorimetric assay was also established for the accurate detection of ASFV in clinical samples. Taken together, we developed a rapid, instrument-free, and low-cost Exo III-assisted RAA colorimetric-assay-based nucleic acid detection platform.
 
Date 2023
2023-09-21T11:55:23Z
2023-09-21T11:55:23Z
 
Type Journal Article
 
Identifier Zhou, Y., Zhang, J., Sun, H., Tao, D., Xu, B., Han, X., Ren, R., Ruan, J., Steinaa, L., Hemmink, J.D., Han, J., Li, X., Xu, J., Zhao, S., Xie, S. and Zhao, C. 2023. Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids. ACS Synthetic Biology.
2161-5063
https://hdl.handle.net/10568/131922
https://doi.org/10.1021/acssynbio.3c00137
 
Language en
 
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Source ACS Synthetic Biology