Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids
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Title |
Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids
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Creator |
Zhou, Y.
Zhang, J. Sun, H. Tao, D. Xu, B. Han, X. Ren, R. Ruan, J. Steinaa, Lucilla Hemmink, Johanneke D. Han Jianlin Li, X. Xu, J. Zhao, S. Xie, S. Zhao, C. |
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Subject |
african swine fever virus
diagnostic techniques |
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Description |
The development of a contamination-free and on-site nucleic acid detection platform with high sensitivity and specificity but low-cost for the detection of pathogenic nucleic acids is critical for infectious disease diagnosis and surveillance. In this study, we combined the recombinase-aided amplification (RAA) with the exonuclease III (Exo III)-assisted signal amplification into a platform for sensitive and specific detection of nucleic acids of African swine fever virus (ASFV). We found that this platform enabled a naked eye visual detection of ASFV at a detection limit as low as 2 copies/μL in 30 min. As expected, no cross-reactivity was observed with other porcine viruses. In addition, to avoid aerosol contamination, a one-tube RAA-Exo III colorimetric assay was also established for the accurate detection of ASFV in clinical samples. Taken together, we developed a rapid, instrument-free, and low-cost Exo III-assisted RAA colorimetric-assay-based nucleic acid detection platform.
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Date |
2023
2023-09-21T11:55:23Z 2023-09-21T11:55:23Z |
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Type |
Journal Article
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Identifier |
Zhou, Y., Zhang, J., Sun, H., Tao, D., Xu, B., Han, X., Ren, R., Ruan, J., Steinaa, L., Hemmink, J.D., Han, J., Li, X., Xu, J., Zhao, S., Xie, S. and Zhao, C. 2023. Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids. ACS Synthetic Biology.
2161-5063 https://hdl.handle.net/10568/131922 https://doi.org/10.1021/acssynbio.3c00137 |
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Language |
en
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Rights |
Copyrighted; all rights reserved
Limited Access |
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Source |
ACS Synthetic Biology
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