Record Details

Administration of Curcumin, Betanin, and CoQ10 combined with nickel oxide, iron superoxide nanoparticles show preventive effects in breast cancer: Effect on apoptosis pathway and MiR-455 expression

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Administration of Curcumin, Betanin, and CoQ10 combined with nickel oxide, iron superoxide nanoparticles show preventive effects in breast cancer: Effect on apoptosis pathway and MiR-455 expression
 
Creator Ganjipour, Ghazaleh
Heshmati, Masomeh
Hashemi, Mehrdad
Entezari, Maliheh
 
Subject Betanin
Breast cancer
Coenzyme Q10
Curcumin
miRNA
Nanoparticles
 
Description 790-802
In the present study, we aimed to evaluate the cytotoxicity and antitumor effect of alone and combined treatment of
curcumin, betanin, and coenzyme Q10 (CoQ10) compounds and nickel oxide (NiO) and iron superoxide (Fe2O3) nanoparticles
(NPs) on breast cancer cells in vitro and in vivo. The 4T1 breast cancer cells were exposed to different concentrations of
Q10, NiO, and Fe2O3 NPs and the inhibitory concentration (IC50) of NPs and compounds and their effect on cell viability
was evaluated by MTT assay. Apoptosis induction in BALB/c mice after treatment with the IC50 concentration of tested
compounds was evaluated by flow cytometry. Gene expression was measured using quantitative real-time polymerase chain
reaction (qRT-PCR). The IC50 values for Fe2O3 and NiO NPs were found to be 92.42 μg/mL and 21.49 μg/mL, respectively,
and were 0.87 μg/mL, 60.14 μg/mL and 83.47 μg/mL for curcumin, betanin, and CoQ10, respectively. Curcumin was more
cytotoxic, whereas Fe2O3 showed lower cytotoxicity than the other compounds in the 4T1 line. All treatments significantly
exerted anticancer activity against breast tumors. qRT-PCR analysis revealed that treatment with IC50 concentrations of all
alone and combined compounds downregulated the expression of Bcl2 and upregulated Bax in breast tumor. The results
revealed a significant reduction in TFAM and MiR-455 expression levels. The combination of aforementioned antitumor agents
with Fe2O3 and NiO NPs shows a synergistic impact, as apoptosis induction is boosted by a combination of antitumor agents
and NPs, and a higher regulatory impact on gene expression occurs compared with monotherapy.
 
Date 2023-09-27T11:56:56Z
2023-09-27T11:56:56Z
2023-09
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/62597
https://doi.org/10.56042/ijbb.v60i10.2804
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBB Vol.60(10) [October 2023]